{"id":130,"date":"2023-03-20T13:45:37","date_gmt":"2023-03-20T12:45:37","guid":{"rendered":"https:\/\/mu-imagine.com\/?page_id=130"},"modified":"2024-06-21T16:15:29","modified_gmt":"2024-06-21T14:15:29","slug":"techniques","status":"publish","type":"page","link":"https:\/\/mu-imagine.com\/techniques\/","title":{"rendered":"Techniques"},"content":{"rendered":"

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How to do aberration correction
<\/span>for various microscopy techniques<\/h1>\n

[\/et_pb_text][\/et_pb_column][\/et_pb_row][et_pb_row column_structure=”1_6,1_6,1_6,1_2″ _builder_version=”4.23.1″ _module_preset=”default” max_width=”1400px” custom_padding=”0px||0px|||” locked=”off” global_colors_info=”{}”][et_pb_column type=”1_6″ _builder_version=”4.23.1″ _module_preset=”default” global_colors_info=”{}”][et_pb_blurb title=”SMLM” image=”https:\/\/mu-imagine.com\/wp-content\/uploads\/2024\/04\/SMLM_technique.png” alt=”SMLM cell icon” image_icon_width=”115px” content_max_width=”100%” module_class=”header-icons” _builder_version=”4.23.1″ _module_preset=”default” header_level=”h3″ header_font=”Montserrat|600|||||||” header_text_align=”center” header_text_color=”#FFFFFF” header_font_size=”16px” body_font=”Montserrat||||||||” body_text_align=”center” body_text_color=”#FFFFFF” image_icon_custom_margin=”0px||0px||false|false” image_icon_custom_margin_tablet=”0px||0px||false|false” image_icon_custom_margin_phone=”5px||||false|false” image_icon_custom_margin_last_edited=”on|phone” animation=”off” link_option_url=”#smlm” locked=”off” global_colors_info=”{}”][\/et_pb_blurb][\/et_pb_column][et_pb_column type=”1_6″ _builder_version=”4.23.1″ _module_preset=”default” global_colors_info=”{}”][et_pb_blurb title=”Light-sheet” image=”https:\/\/mu-imagine.com\/wp-content\/uploads\/2024\/04\/Light-sheet_technique.png” alt=”Light-sheet objectives icon” image_icon_width=”115px” content_max_width=”100%” module_class=”header-icons” _builder_version=”4.23.1″ _module_preset=”default” header_level=”h3″ header_font=”Montserrat|600|||||||” header_text_align=”center” header_text_color=”#FFFFFF” header_font_size=”16px” body_font=”Montserrat||||||||” body_text_align=”center” body_text_color=”#FFFFFF” image_icon_custom_margin=”||0px||false|false” animation=”off” link_option_url=”#light” locked=”off” global_colors_info=”{}”][\/et_pb_blurb][\/et_pb_column][et_pb_column type=”1_6″ _builder_version=”4.23.1″ _module_preset=”default” global_colors_info=”{}”][et_pb_blurb title=”Multiphoton” image=”https:\/\/mu-imagine.com\/wp-content\/uploads\/2024\/04\/Multiphoton_technique.png” alt=”Multiphoton objective icon” image_icon_width=”115px” content_max_width=”100%” module_class=”header-icons” _builder_version=”4.23.1″ _module_preset=”default” header_level=”h3″ header_font=”Montserrat|600|||||||” header_text_align=”center” header_text_color=”#FFFFFF” header_font_size=”16px” body_font=”Montserrat||||||||” body_text_align=”center” body_text_color=”#FFFFFF” image_icon_custom_margin=”0px||0px||false|false” image_icon_custom_margin_tablet=”0px||0px||false|false” image_icon_custom_margin_phone=”-12px||||false|false” image_icon_custom_margin_last_edited=”on|phone” width=”100%” custom_margin_tablet=”” custom_margin_phone=”6px||0px||false|false” custom_margin_last_edited=”on|tablet” animation=”off” link_option_url=”#multi” locked=”off” global_colors_info=”{}”][\/et_pb_blurb][\/et_pb_column][et_pb_column type=”1_2″ _builder_version=”4.23.1″ _module_preset=”default” global_colors_info=”{}”][\/et_pb_column][\/et_pb_row][\/et_pb_section][et_pb_section fb_built=”1″ disabled_on=”off|off|off” module_id=”smlm” _builder_version=”4.23.1″ _module_preset=”default” background_color=”#cbe9fb” background_enable_image=”off” hover_enabled=”0″ global_colors_info=”{}” sticky_enabled=”0″][et_pb_row use_custom_gutter=”on” gutter_width=”2″ _builder_version=”4.23.1″ _module_preset=”default” max_width=”1400px” custom_padding=”0px||0px||true|false” custom_css_main_element=”display: flex;” global_colors_info=”{}” custom_css_main_element_last_edited=”on|phone” custom_css_main_element_phone=”display: block;” custom_css_main_element_tablet=”display: flex;”][et_pb_column type=”4_4″ _builder_version=”4.20.1″ _module_preset=”default” global_colors_info=”{}”][et_pb_heading title=”Single Molecule Localization Microscopy” _builder_version=”4.23.1″ _module_preset=”default” title_level=”h2″ title_font=”Montserrat|700|||||||” title_text_color=”#000000″ title_font_size=”25px” custom_margin=”||15px||false|false” locked=”off” global_colors_info=”{}”][\/et_pb_heading][\/et_pb_column][\/et_pb_row][et_pb_row column_structure=”1_2,1_2″ use_custom_gutter=”on” gutter_width=”2″ make_equal=”on” _builder_version=”4.23.1″ _module_preset=”default” max_width=”1400px” custom_padding=”0px||0px||true|false” custom_css_main_element=”display: flex;||align-items: center;” global_colors_info=”{}” custom_css_main_element_last_edited=”on|tablet” custom_css_main_element_phone=”display: block;||” custom_css_main_element_tablet=”display: flex;||align-items: center;”][et_pb_column type=”1_2″ _builder_version=”4.20.1″ _module_preset=”default” custom_css_main_element=”margin: auto;” global_colors_info=”{}”][et_pb_text _builder_version=”4.23.1″ _module_preset=”default” text_font=”Montserrat||||||||” text_text_color=”#000000″ text_font_size=”13px” header_3_font=”Montserrat|700|||||||” header_3_text_color=”#FFFFFF” header_3_font_size=”25px” custom_margin=”||20px||false|false” header_3_font_size_tablet=”25px” header_3_font_size_phone=”22px” header_3_font_size_last_edited=”on|phone” global_colors_info=”{}”]<\/p>\n

For this thechnique, adaptive optics will help you to :<\/p>\n

[\/et_pb_text][et_pb_blurb use_icon=”on” font_icon=”R||divi||400″ icon_color=”#1b375c” icon_placement=”left” image_icon_width=”22px” content_max_width=”100%” _builder_version=”4.23.1″ _module_preset=”default” body_font=”Montserrat||||||||” body_text_color=”#000000″ body_font_size=”13px” body_line_height=”1.3em” animation=”off” global_colors_info=”{}”]<\/p>\n

Correct aberrations<\/p>\n

[\/et_pb_blurb][et_pb_blurb use_icon=”on” font_icon=”R||divi||400″ icon_color=”#1b375c” icon_placement=”left” image_icon_width=”22px” content_max_width=”100%” _builder_version=”4.23.1″ _module_preset=”default” body_font=”Montserrat||||||||” body_text_color=”#000000″ body_font_size=”13px” body_line_height=”1.3em” animation=”off” global_colors_info=”{}”]<\/p>\n

Increase signal<\/p>\n

[\/et_pb_blurb][et_pb_blurb use_icon=”on” font_icon=”R||divi||400″ icon_color=”#1b375c” icon_placement=”left” image_icon_width=”22px” content_max_width=”100%” _builder_version=”4.23.1″ _module_preset=”default” body_font=”Montserrat||||||||” body_text_color=”#000000″ body_font_size=”13px” body_line_height=”1.3em” animation=”off” global_colors_info=”{}”]<\/p>\n

Go 3D<\/p>\n

[\/et_pb_blurb][et_pb_button button_url=”@ET-DC@eyJkeW5hbWljIjp0cnVlLCJjb250ZW50IjoicG9zdF9saW5rX3VybF9wYWdlIiwic2V0dGluZ3MiOnsicG9zdF9pZCI6IjM2MCJ9fQ==@” button_text=”Go to SMLM page” button_alignment=”left” button_alignment_tablet=”center” button_alignment_phone=”center” button_alignment_last_edited=”off|desktop” disabled_on=”off|off|off” _builder_version=”4.23.1″ _dynamic_attributes=”button_url” _module_preset=”default” custom_button=”on” button_text_size=”16px” button_text_color=”#000000″ button_bg_color=”#0cb8dc” button_border_width=”0px” button_border_color=”#50D3E1″ button_border_radius=”100px” button_font=”Montserrat|600|||||||” button_icon=”$||divi||400″ button_icon_color=”#000000″ custom_margin=”35px||||false|false” custom_margin_tablet=”35px||||false|false” custom_margin_phone=”35px||35px||false|false” custom_margin_last_edited=”on|phone” locked=”off” global_colors_info=”{}”][\/et_pb_button][\/et_pb_column][et_pb_column type=”1_2″ _builder_version=”4.20.1″ _module_preset=”default” global_colors_info=”{}”][et_pb_image src=”https:\/\/mu-imagine.com\/wp-content\/uploads\/2024\/04\/SMLM_techniques.png” alt=”Image of aberration correction for SMLM with cylindrical lens vs with adaptive optics” title_text=”smlm-example_aberration-correction-tech” _builder_version=”4.23.1″ _module_preset=”default” max_width=”75%” module_alignment=”center” box_shadow_style=”preset1″ global_colors_info=”{}”][\/et_pb_image][\/et_pb_column][\/et_pb_row][\/et_pb_section][et_pb_section fb_built=”1″ disabled_on=”off|off|off” module_id=”light” _builder_version=”4.23.1″ _module_preset=”default” background_color=”#cbe9fb” background_enable_image=”off” hover_enabled=”0″ global_colors_info=”{}” sticky_enabled=”0″][et_pb_row use_custom_gutter=”on” gutter_width=”2″ _builder_version=”4.23.1″ _module_preset=”default” max_width=”1400px” custom_padding=”0px||0px||true|false” custom_css_main_element=”display: flex;” global_colors_info=”{}” custom_css_main_element_last_edited=”on|phone” custom_css_main_element_phone=”display: block;” custom_css_main_element_tablet=”display: flex;”][et_pb_column type=”4_4″ _builder_version=”4.20.1″ _module_preset=”default” global_colors_info=”{}”][et_pb_heading title=”Lattice & Light-sheet microscopy” _builder_version=”4.23.1″ _module_preset=”default” title_level=”h2″ title_font=”Montserrat|700|||||||” title_text_color=”#000000″ title_font_size=”25px” custom_margin=”||15px||false|false” locked=”off” global_colors_info=”{}”][\/et_pb_heading][\/et_pb_column][\/et_pb_row][et_pb_row column_structure=”1_2,1_2″ use_custom_gutter=”on” gutter_width=”2″ make_equal=”on” _builder_version=”4.23.1″ _module_preset=”default” max_width=”1400px” custom_padding=”0px||0px||true|false” custom_css_main_element=”display: flex;||align-items: center;” global_colors_info=”{}” custom_css_main_element_last_edited=”on|phone” custom_css_main_element_phone=”display: block;||” custom_css_main_element_tablet=”display: flex;||align-items: center;”][et_pb_column type=”1_2″ _builder_version=”4.20.1″ _module_preset=”default” custom_css_main_element=”margin: auto;” global_colors_info=”{}”][et_pb_text _builder_version=”4.23.1″ _module_preset=”default” text_font=”Montserrat||||||||” text_text_color=”#000000″ text_font_size=”13px” header_3_font=”Montserrat|700|||||||” header_3_text_color=”#FFFFFF” header_3_font_size=”25px” custom_margin=”||20px||false|false” header_3_font_size_tablet=”25px” header_3_font_size_phone=”22px” header_3_font_size_last_edited=”on|phone” global_colors_info=”{}”]<\/p>\n

For this thechnique, adaptive optics will help you to :<\/p>\n

[\/et_pb_text][et_pb_blurb use_icon=”on” font_icon=”R||divi||400″ icon_color=”#1b375c” icon_placement=”left” image_icon_width=”22px” content_max_width=”100%” _builder_version=”4.23.1″ _module_preset=”default” body_font=”Montserrat||||||||” body_text_color=”#000000″ body_font_size=”13px” body_line_height=”1.3em” animation=”off” global_colors_info=”{}”]<\/p>\n

Correct aberrations<\/p>\n

[\/et_pb_blurb][et_pb_blurb use_icon=”on” font_icon=”R||divi||400″ icon_color=”#1b375c” icon_placement=”left” image_icon_width=”22px” content_max_width=”100%” _builder_version=”4.23.1″ _module_preset=”default” body_font=”Montserrat||||||||” body_text_color=”#000000″ body_font_size=”13px” body_line_height=”1.3em” animation=”off” global_colors_info=”{}”]<\/p>\n

Improve cell resolution<\/p>\n

[\/et_pb_blurb][et_pb_blurb use_icon=”on” font_icon=”R||divi||400″ icon_color=”#1b375c” icon_placement=”left” image_icon_width=”22px” content_max_width=”100%” _builder_version=”4.23.1″ _module_preset=”default” body_font=”Montserrat||||||||” body_text_color=”#000000″ body_font_size=”13px” body_line_height=”1.3em” animation=”off” global_colors_info=”{}”]<\/p>\n

Image deeper<\/p>\n

[\/et_pb_blurb][et_pb_button button_url=”@ET-DC@eyJkeW5hbWljIjp0cnVlLCJjb250ZW50IjoicG9zdF9saW5rX3VybF9wYWdlIiwic2V0dGluZ3MiOnsicG9zdF9pZCI6IjM0MiJ9fQ==@” button_text=”Go to Light-sheet page” button_alignment=”left” button_alignment_tablet=”center” button_alignment_phone=”center” button_alignment_last_edited=”off|desktop” disabled_on=”off|off|off” _builder_version=”4.23.1″ _dynamic_attributes=”button_url” _module_preset=”default” custom_button=”on” button_text_size=”16px” button_text_color=”#000000″ button_bg_color=”#0cb8dc” button_border_width=”0px” button_border_color=”#50D3E1″ button_border_radius=”100px” button_font=”Montserrat|600|||||||” button_icon=”$||divi||400″ button_icon_color=”#000000″ custom_margin=”35px||||false|false” custom_margin_tablet=”35px||||false|false” custom_margin_phone=”35px||35px||false|false” custom_margin_last_edited=”on|phone” locked=”off” global_colors_info=”{}”][\/et_pb_button][\/et_pb_column][et_pb_column type=”1_2″ _builder_version=”4.20.1″ _module_preset=”default” global_colors_info=”{}”][et_pb_image src=”https:\/\/mu-imagine.com\/wp-content\/uploads\/2024\/04\/Light-sheet_techniques.png” alt=”Image of aberration correction for light-sheet with vs without adaptive optics” title_text=”light-sheet_aberration-correction-tech” _builder_version=”4.23.1″ _module_preset=”default” max_width=”75%” module_alignment=”center” box_shadow_style=”preset1″ global_colors_info=”{}”][\/et_pb_image][\/et_pb_column][\/et_pb_row][\/et_pb_section][et_pb_section fb_built=”1″ disabled_on=”off|off|off” module_id=”multi” _builder_version=”4.23.1″ _module_preset=”default” background_color=”#cbe9fb” background_enable_image=”off” bottom_divider_style=”wave” bottom_divider_color=”#0e0a0f” bottom_divider_height=”70px” hover_enabled=”0″ global_colors_info=”{}” sticky_enabled=”0″][et_pb_row use_custom_gutter=”on” gutter_width=”2″ _builder_version=”4.23.1″ _module_preset=”default” max_width=”1400px” custom_padding=”0px||0px||true|false” custom_css_main_element=”display: flex;” global_colors_info=”{}” custom_css_main_element_last_edited=”on|phone” custom_css_main_element_phone=”display: block;” custom_css_main_element_tablet=”display: flex;”][et_pb_column type=”4_4″ _builder_version=”4.20.1″ _module_preset=”default” global_colors_info=”{}”][et_pb_heading title=”Multiphoton microscopy” _builder_version=”4.23.1″ _module_preset=”default” title_level=”h2″ title_font=”Montserrat|700|||||||” title_text_color=”#000000″ title_font_size=”25px” custom_margin=”||15px||false|false” locked=”off” global_colors_info=”{}”][\/et_pb_heading][\/et_pb_column][\/et_pb_row][et_pb_row column_structure=”1_2,1_2″ use_custom_gutter=”on” gutter_width=”2″ make_equal=”on” _builder_version=”4.23.1″ _module_preset=”default” max_width=”1400px” custom_padding=”0px||0px||true|false” custom_css_main_element=”display: flex;||align-items: center;” global_colors_info=”{}” custom_css_main_element_last_edited=”on|phone” custom_css_main_element_phone=”display: block;||” custom_css_main_element_tablet=”display: flex;||align-items: center;”][et_pb_column type=”1_2″ _builder_version=”4.20.1″ _module_preset=”default” custom_css_main_element=”margin: auto;” global_colors_info=”{}”][et_pb_text _builder_version=”4.23.1″ _module_preset=”default” text_font=”Montserrat||||||||” text_text_color=”#000000″ text_font_size=”13px” header_3_font=”Montserrat|700|||||||” header_3_text_color=”#FFFFFF” header_3_font_size=”25px” custom_margin=”||20px||false|false” header_3_font_size_tablet=”25px” header_3_font_size_phone=”22px” header_3_font_size_last_edited=”on|phone” global_colors_info=”{}”]<\/p>\n

For this thechnique, adaptive optics will help you to :<\/p>\n

[\/et_pb_text][et_pb_blurb use_icon=”on” font_icon=”R||divi||400″ icon_color=”#1b375c” icon_placement=”left” image_icon_width=”22px” content_max_width=”100%” _builder_version=”4.23.1″ _module_preset=”default” body_font=”Montserrat||||||||” body_text_color=”#000000″ body_font_size=”13px” body_line_height=”1.3em” animation=”off” global_colors_info=”{}”]<\/p>\n

Correct aberrations<\/p>\n

[\/et_pb_blurb][et_pb_blurb use_icon=”on” font_icon=”R||divi||400″ icon_color=”#1b375c” icon_placement=”left” image_icon_width=”22px” content_max_width=”100%” _builder_version=”4.23.1″ _module_preset=”default” body_font=”Montserrat||||||||” body_text_color=”#000000″ body_font_size=”13px” body_line_height=”1.3em” animation=”off” global_colors_info=”{}”]<\/p>\n

Boost fluorescence<\/p>\n

[\/et_pb_blurb][et_pb_blurb use_icon=”on” font_icon=”R||divi||400″ icon_color=”#1b375c” icon_placement=”left” image_icon_width=”22px” content_max_width=”100%” _builder_version=”4.23.1″ _module_preset=”default” body_font=”Montserrat||||||||” body_text_color=”#000000″ body_font_size=”13px” body_line_height=”1.3em” animation=”off” global_colors_info=”{}”]<\/p>\n

Image deeper<\/p>\n

[\/et_pb_blurb][et_pb_button button_url=”@ET-DC@eyJkeW5hbWljIjp0cnVlLCJjb250ZW50IjoicG9zdF9saW5rX3VybF9wYWdlIiwic2V0dGluZ3MiOnsicG9zdF9pZCI6IjM1MiJ9fQ==@” button_text=”Go to Multiphoton page” button_alignment=”left” button_alignment_tablet=”center” button_alignment_phone=”center” button_alignment_last_edited=”off|desktop” disabled_on=”off|off|off” _builder_version=”4.23.1″ _dynamic_attributes=”button_url” _module_preset=”default” custom_button=”on” button_text_size=”16px” button_text_color=”#000000″ button_bg_color=”#0cb8dc” button_border_width=”0px” button_border_color=”#50D3E1″ button_border_radius=”100px” button_font=”Montserrat|600|||||||” button_icon=”$||divi||400″ button_icon_color=”#000000″ custom_margin=”35px||||false|false” custom_margin_tablet=”||20px||false|false” custom_margin_phone=”35px||35px||false|false” custom_margin_last_edited=”on|phone” locked=”off” global_colors_info=”{}”][\/et_pb_button][\/et_pb_column][et_pb_column type=”1_2″ _builder_version=”4.20.1″ _module_preset=”default” global_colors_info=”{}”][et_pb_image src=”https:\/\/mu-imagine.com\/wp-content\/uploads\/2024\/04\/Multiphoton_techniques.png” alt=”Image of aberration correction for multiphoton with vs without adaptive optics” title_text=”multiphoton_aberration-correction-tech” _builder_version=”4.23.1″ _module_preset=”default” max_width=”75%” module_alignment=”center” custom_margin_tablet=”” custom_margin_phone=”||35px||false|false” custom_margin_last_edited=”on|phone” box_shadow_style=”preset1″ global_colors_info=”{}”][\/et_pb_image][\/et_pb_column][\/et_pb_row][\/et_pb_section]<\/p>\n","protected":false},"excerpt":{"rendered":"

How to do aberration correctionfor various microscopy techniquesFor this thechnique, adaptive optics will help you to :Correct aberrationsIncrease signalGo 3DFor this thechnique, adaptive optics will help you to :Correct aberrationsImprove cell resolutionImage deeperFor this thechnique, adaptive optics will help you to :Correct aberrationsBoost fluorescenceImage deeper<\/p>\n","protected":false},"author":1,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_et_pb_use_builder":"on","_et_pb_old_content":"","_et_gb_content_width":"","footnotes":""},"dipi_cpt_category":[],"class_list":["post-130","page","type-page","status-publish","hentry"],"yoast_head":"\nAberration correction in microscopy | Techniques<\/title>\n<meta name=\"description\" content=\"Discover more about the benefits of aberration correction in microscopy (overview of SMLM, light-sheet and multiphoton techniques).\" \/>\n<meta name=\"robots\" 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