{"id":342,"date":"2023-03-30T12:39:57","date_gmt":"2023-03-30T10:39:57","guid":{"rendered":"https:\/\/mu-imagine.com\/?page_id=342"},"modified":"2024-06-21T16:50:24","modified_gmt":"2024-06-21T14:50:24","slug":"light-sheet-microscopy-overview","status":"publish","type":"page","link":"https:\/\/mu-imagine.com\/techniques\/light-sheet-microscopy-overview\/","title":{"rendered":"Light-sheet"},"content":{"rendered":"

[et_pb_section fb_built=”1″ disabled_on=”off|off|off” _builder_version=”4.23.1″ _module_preset=”default” background_image=”https:\/\/mu-imagine.com\/wp-content\/uploads\/2024\/04\/Light-sheet-background.png” bottom_divider_style=”wave” bottom_divider_color=”#cbe9fb” bottom_divider_height=”70px” bottom_divider_flip=”horizontal” hover_enabled=”0″ locked=”off” global_colors_info=”{}” sticky_enabled=”0″][et_pb_row column_structure=”1_2,1_2″ _builder_version=”4.23.1″ _module_preset=”default” max_width=”1400px” custom_margin=”|auto|33px|auto||” custom_padding=”||0px|||” locked=”off” global_colors_info=”{}”][et_pb_column type=”1_2″ _builder_version=”4.23.1″ _module_preset=”default” global_colors_info=”{}”][\/et_pb_column][et_pb_column type=”1_2″ _builder_version=”4.23.1″ _module_preset=”default” global_colors_info=”{}”][et_pb_text _builder_version=”4.23.1″ _module_preset=”default” text_font=”Montserrat||||||||” text_text_color=”#FFFFFF” header_font=”Montserrat|700|||||||” header_text_color=”#cbe9fb” header_font_size=”33px” global_colors_info=”{}”]<\/p>\n

Light-sheet microscopy,
<\/span>a brief overview<\/h1>\n

[\/et_pb_text][\/et_pb_column][\/et_pb_row][et_pb_row column_structure=”1_6,1_6,1_6,1_2″ _builder_version=”4.23.1″ _module_preset=”default” max_width=”1400px” custom_padding=”0px||0px|||” locked=”off” global_colors_info=”{}”][et_pb_column type=”1_6″ _builder_version=”4.23.1″ _module_preset=”default” global_colors_info=”{}”][et_pb_blurb title=”Correct” image=”https:\/\/mu-imagine.com\/wp-content\/uploads\/2024\/04\/Correct_light-sheet.png” alt=”PSF correction icon” image_icon_width=”115px” content_max_width=”100%” module_class=”header-icons” _builder_version=”4.23.1″ _module_preset=”default” header_level=”h3″ header_font=”Montserrat|600|||||||” header_text_align=”center” header_text_color=”#FFFFFF” header_font_size=”16px” body_font=”Montserrat||||||||” body_text_align=”center” body_text_color=”#FFFFFF” body_font_size=”13px” body_line_height=”1em” image_icon_custom_margin=”0px||0px||false|false” image_icon_custom_margin_tablet=”0px||0px||false|false” image_icon_custom_margin_phone=”5px||||false|false” image_icon_custom_margin_last_edited=”on|phone” animation=”off” locked=”off” global_colors_info=”{}”]<\/p>\n

aberrations<\/p>\n

[\/et_pb_blurb][\/et_pb_column][et_pb_column type=”1_6″ _builder_version=”4.23.1″ _module_preset=”default” global_colors_info=”{}”][et_pb_blurb title=”Improve” image=”https:\/\/mu-imagine.com\/wp-content\/uploads\/2024\/04\/Resolution_light-sheet.png” alt=”Resolution improvement icon” image_icon_width=”115px” content_max_width=”100%” module_class=”header-icons” _builder_version=”4.23.1″ _module_preset=”default” header_level=”h3″ header_font=”Montserrat|600|||||||” header_text_align=”center” header_text_color=”#FFFFFF” header_font_size=”16px” body_font=”Montserrat||||||||” body_text_align=”center” body_text_color=”#FFFFFF” body_font_size=”13px” body_line_height=”1em” image_icon_custom_margin=”||0px||false|false” animation=”off” locked=”off” global_colors_info=”{}”]<\/p>\n

resolution<\/p>\n

[\/et_pb_blurb][\/et_pb_column][et_pb_column type=”1_6″ _builder_version=”4.23.1″ _module_preset=”default” global_colors_info=”{}”][et_pb_blurb title=”Image” image=”https:\/\/mu-imagine.com\/wp-content\/uploads\/2024\/04\/Deep_light-sheet.png” alt=”In depth imaging icon” image_icon_width=”115px” content_max_width=”100%” module_class=”header-icons” _builder_version=”4.23.1″ _module_preset=”default” header_level=”h3″ header_font=”Montserrat|600|||||||” header_text_align=”center” header_text_color=”#FFFFFF” header_font_size=”16px” body_font=”Montserrat||||||||” body_text_align=”center” body_text_color=”#FFFFFF” body_font_size=”13px” body_line_height=”1em” image_icon_custom_margin=”0px||0px||false|false” image_icon_custom_margin_tablet=”0px||0px||false|false” image_icon_custom_margin_phone=”-12px||||false|false” image_icon_custom_margin_last_edited=”on|phone” width=”100%” custom_margin_tablet=”” custom_margin_phone=”-6px||0px||false|false” custom_margin_last_edited=”on|tablet” animation=”off” locked=”off” global_colors_info=”{}”]<\/p>\n

deeper<\/p>\n

[\/et_pb_blurb][\/et_pb_column][et_pb_column type=”1_2″ _builder_version=”4.23.1″ _module_preset=”default” global_colors_info=”{}”][\/et_pb_column][\/et_pb_row][\/et_pb_section][et_pb_section fb_built=”1″ disabled_on=”off|off|off” _builder_version=”4.23.1″ _module_preset=”default” background_color=”#cbe9fb” background_enable_image=”off” background_pattern_repeat_origin=”center” hover_enabled=”0″ locked=”off” global_colors_info=”{}” sticky_enabled=”0″][et_pb_row _builder_version=”4.23.1″ _module_preset=”default” max_width=”1400px” custom_padding=”0px|||||” locked=”off” global_colors_info=”{}”][et_pb_column type=”4_4″ _builder_version=”4.23.1″ _module_preset=”default” global_colors_info=”{}”][et_pb_heading title=”About the technique” _builder_version=”4.23.1″ _module_preset=”default” title_level=”h2″ title_font=”Montserrat|700|||||||” title_text_color=”#000000″ title_font_size=”25px” custom_margin=”||-20px||false|false” custom_margin_tablet=”||-30px||false|false” custom_margin_phone=”||-30px||false|false” custom_margin_last_edited=”on|tablet” global_colors_info=”{}”][\/et_pb_heading][\/et_pb_column][\/et_pb_row][et_pb_row column_structure=”1_2,1_2″ use_custom_gutter=”on” gutter_width=”2″ make_equal=”on” _builder_version=”4.23.1″ _module_preset=”default” max_width=”1400px” custom_css_main_element=”display: flex;||align-items: center;” global_colors_info=”{}” custom_css_main_element_last_edited=”on|phone” custom_css_main_element_phone=”display: block;” custom_css_main_element_tablet=”display: block;”][et_pb_column type=”1_2″ _builder_version=”4.20.1″ _module_preset=”default” custom_css_main_element=”margin: auto;” global_colors_info=”{}”][et_pb_text _builder_version=”4.23.1″ _module_preset=”default” text_font=”Montserrat||||||||” text_text_color=”#000000″ text_font_size=”13px” header_3_font=”Montserrat|700|||||||” header_3_text_color=”#FFFFFF” header_3_font_size=”25px” custom_margin=”||35px||false|false” header_3_font_size_tablet=”25px” header_3_font_size_phone=”22px” header_3_font_size_last_edited=”on|phone” global_colors_info=”{}”]<\/p>\n

Various light-sheet imaging techniques differ in the way the light-sheet is created,
however, they all face the same problematics regarding aberrations.<\/p>\n

These techniques are typically used to do live imaging of optically inhomogenious
biological samples. A number of refractive index changes are then causing aberrations.<\/p>\n

This impacts PSF quality, which ultimatly will decrease image contrast.<\/p>\n

[\/et_pb_text][\/et_pb_column][et_pb_column type=”1_2″ _builder_version=”4.20.1″ _module_preset=”default” global_colors_info=”{}”][et_pb_image src=”https:\/\/mu-imagine.com\/wp-content\/uploads\/2024\/04\/no-AO_Light-sheet.png” alt=”Examples of light-sheet and lattice light-sheet imaging without adaptive optics” title_text=”no-ao_light-sheet” align=”center” _builder_version=”4.23.1″ _module_preset=”default” max_width=”75%” box_shadow_style=”preset1″ global_colors_info=”{}”][\/et_pb_image][\/et_pb_column][\/et_pb_row][\/et_pb_section][et_pb_section fb_built=”1″ disabled_on=”off|off|off” _builder_version=”4.23.1″ _module_preset=”default” background_color=”#cbe9fb” hover_enabled=”0″ locked=”off” global_colors_info=”{}” sticky_enabled=”0″][et_pb_row _builder_version=”4.23.1″ _module_preset=”default” max_width=”1400px” custom_margin=”|auto|-32px|auto||” custom_padding=”0px|||||” locked=”off” global_colors_info=”{}”][et_pb_column type=”4_4″ _builder_version=”4.23.1″ _module_preset=”default” global_colors_info=”{}”][et_pb_heading title=”What can AO bring ?” _builder_version=”4.23.1″ _module_preset=”default” title_level=”h2″ title_font=”Montserrat|700|||||||” title_text_color=”#000000″ title_font_size=”25px” custom_margin=”||-25px||false|false” custom_margin_tablet=”||35px||false|false” custom_margin_phone=”||35px||false|false” custom_margin_last_edited=”on|desktop” locked=”off” global_colors_info=”{}”][\/et_pb_heading][\/et_pb_column][\/et_pb_row][et_pb_row column_structure=”1_2,1_2″ make_equal=”on” _builder_version=”4.23.1″ _module_preset=”default” max_width=”1400px” custom_margin=”|auto|-65px|auto|false|false” custom_padding=”0px||0px||false|false” custom_css_main_element=”align-items: center;” global_colors_info=”{}”][et_pb_column type=”1_2″ _builder_version=”4.23.1″ _module_preset=”default” global_colors_info=”{}”][et_pb_blurb use_icon=”on” font_icon=”R||divi||400″ icon_color=”#02486A” icon_placement=”left” image_icon_width=”22px” content_max_width=”100%” _builder_version=”4.23.1″ _module_preset=”default” body_font=”Montserrat||||||||” body_text_color=”#000000″ body_font_size=”13px” body_line_height=”1.3em” custom_margin=”||10px||false|false” animation=”off” locked=”off” global_colors_info=”{}”]<\/p>\n

Implement on emission pathway<\/b>, increase fluorescence signal and contrast of the image<\/p>\n

[\/et_pb_blurb][et_pb_blurb use_icon=”on” font_icon=”R||divi||400″ icon_color=”#02486A” icon_placement=”left” image_icon_width=”22px” content_max_width=”100%” _builder_version=”4.23.1″ _module_preset=”default” body_font=”Montserrat||||||||” body_text_color=”#000000″ body_font_size=”13px” body_line_height=”1.3em” custom_margin=”||10px||false|false” animation=”off” locked=”off” global_colors_info=”{}”]<\/p>\n

Correct the system aberrations <\/b>induced by optical elements and sample mounting<\/p>\n

[\/et_pb_blurb][et_pb_blurb use_icon=”on” font_icon=”R||divi||400″ icon_color=”#02486A” icon_placement=”left” image_icon_width=”22px” content_max_width=”100%” _builder_version=”4.23.1″ _module_preset=”default” body_font=”Montserrat||||||||” body_text_color=”#000000″ body_font_size=”13px” body_line_height=”1.3em” custom_margin=”||10px||false|false” animation=”off” locked=”off” global_colors_info=”{}”]<\/p>\n

Correct sample-induced aberrations <\/b>and see biological structures more clearly<\/p>\n

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Related products<\/h4>\n

[\/et_pb_text][dipi_button_grid flex_direction=”row” justify_content=”flex-start” _builder_version=”4.23.1″ _module_preset=”default” text_style_text_color=”#000000″ custom_button_grid=”on” button_grid_text_size=”16px” button_grid_text_color=”#000000″ button_grid_bg_color=”#0cb8dc” button_grid_border_width=”0px” button_grid_font=”Montserrat|600|||||||” hover_enabled=”0″ global_colors_info=”{}” sticky_enabled=”0″][dipi_button_grid_child button_type=”button” button_text=”AOKit Bio” button_link=”@ET-DC@eyJkeW5hbWljIjp0cnVlLCJjb250ZW50IjoicG9zdF9saW5rX3VybF9wYWdlIiwic2V0dGluZ3MiOnsicG9zdF9pZCI6IjIwMiJ9fQ==@” _builder_version=”4.23.1″ _dynamic_attributes=”button_link” _module_preset=”default” custom_button=”on” button_text_size=”16px” button_text_color=”#000000″ button_bg_color=”#0cb8dc” button_border_width=”0px” button_border_color=”#50D3E1″ button_border_radius=”100px” button_font=”Montserrat|600|||||||” button_icon=”$||divi||400″ button_icon_color=”#000000″ button_on_hover=”on” hover_enabled=”0″ global_colors_info=”{}” button_bg_enable_color=”on” sticky_enabled=”0″][\/dipi_button_grid_child][dipi_button_grid_child button_type=”button” button_text=”mu-DM” button_link=”@ET-DC@eyJkeW5hbWljIjp0cnVlLCJjb250ZW50IjoicG9zdF9saW5rX3VybF9wYWdlIiwic2V0dGluZ3MiOnsicG9zdF9pZCI6IjE0NiJ9fQ==@” _builder_version=”4.23.1″ _dynamic_attributes=”button_link” _module_preset=”default” custom_button=”on” button_text_size=”16px” button_text_color=”#000000″ button_bg_color=”#0cb8dc” button_border_width=”0px” button_border_color=”#50D3E1″ button_border_radius=”100px” button_font=”Montserrat|600|||||||” button_icon=”$||divi||400″ button_icon_color=”#000000″ button_on_hover=”on” hover_enabled=”0″ global_colors_info=”{}” button_bg_enable_color=”on” sticky_enabled=”0″][\/dipi_button_grid_child][dipi_button_grid_child button_type=”button” button_text=”Mirao” button_link=”@ET-DC@eyJkeW5hbWljIjp0cnVlLCJjb250ZW50IjoicG9zdF9saW5rX3VybF9wYWdlIiwic2V0dGluZ3MiOnsicG9zdF9pZCI6IjM3MiJ9fQ==@” _builder_version=”4.23.1″ _dynamic_attributes=”button_link” _module_preset=”default” custom_button=”on” button_text_size=”16px” button_text_color=”#000000″ button_bg_color=”#0cb8dc” button_border_radius=”100px” button_font=”Montserrat|600|||||||” button_icon=”$||divi||400″ button_icon_color=”#000000″ button_on_hover=”on” hover_enabled=”0″ global_colors_info=”{}” sticky_enabled=”0″][\/dipi_button_grid_child][\/dipi_button_grid][\/et_pb_column][et_pb_column type=”1_2″ _builder_version=”4.20.1″ _module_preset=”default” global_colors_info=”{}”][\/et_pb_column][\/et_pb_row][\/et_pb_section][et_pb_section fb_built=”1″ disabled_on=”off|off|off” _builder_version=”4.23.1″ _module_preset=”default” background_color=”#cbe9fb” bottom_divider_style=”wave” bottom_divider_color=”#0e0a0f” bottom_divider_height=”70px” hover_enabled=”0″ locked=”off” global_colors_info=”{}” sticky_enabled=”0″][et_pb_row use_custom_gutter=”on” gutter_width=”2″ _builder_version=”4.20.1″ _module_preset=”default” max_width=”1400px” custom_padding=”0px||0px||true|false” custom_css_main_element=”display: flex;” global_colors_info=”{}” custom_css_main_element_tablet=”display: flex;” custom_css_main_element_phone=”display: block;” custom_css_main_element_last_edited=”on|phone”][et_pb_column type=”4_4″ _builder_version=”4.20.1″ _module_preset=”default” global_colors_info=”{}”][et_pb_heading title=”Applications examples” _builder_version=”4.23.1″ _module_preset=”default” title_level=”h2″ title_font=”Montserrat|700|||||||” title_text_color=”#000000″ title_font_size=”25px” locked=”off” global_colors_info=”{}”][\/et_pb_heading][et_pb_text _builder_version=”4.23.1″ _module_preset=”default” text_font=”Montserrat||||||||” text_text_color=”#000000″ text_font_size=”13px” global_colors_info=”{}”]<\/p>\n

Application notes & white papers coming soon<\/p>\n

[\/et_pb_text][et_pb_text _builder_version=”4.23.1″ _module_preset=”default” text_font=”Montserrat||||||||” text_text_color=”#DDE4E6″ text_font_size=”16px” header_3_font=”Montserrat|700|||||||” header_3_text_color=”#50D3E1″ header_3_font_size=”25px” header_4_font=”Montserrat|700|||||||” header_4_text_color=”#1b375c” header_4_font_size=”20px” custom_margin=”35px||35px||false|false” header_3_font_size_tablet=”25px” header_3_font_size_phone=”22px” header_3_font_size_last_edited=”on|phone” header_4_font_size_tablet=”18px” header_4_font_size_phone=”18px” header_4_font_size_last_edited=”off|desktop” locked=”off” global_colors_info=”{}”]<\/p>\n

Selected publications<\/h4>\n

C. Bourgenot, C. D. Saunter, J. M. Taylor, J. M. Girkin & G. D. Love; “3D adaptive optics in a light sheet microscope”; Opt. Express 20, 13252-13261 (2012)<\/a><\/p>\n

K.M. Dean, P. Roudot, E.S. Welf, T. Pohlkamp, G. Garrelts, J. Herz, & R. Fiolka; “Imaging subcellular dynamics with fast and light-efficient volumetrically parallelized microscopy”; Optica 4, 263-271 (2017).<\/a><\/p>\n

A. Hubert, F. Harms, R. Juv\u00e9nal, P. Treimany, X. Levecq, V. Loriette, G. Farkouh, F. Rouyer & A. Fragola; “Adaptive optics light-sheet microscopy based on direct wavefront sensing without any guide star”; Opt. Lett. 44, 2514-2517 (2019)<\/a>[\/et_pb_text][\/et_pb_column][\/et_pb_row][\/et_pb_section]<\/p>\n","protected":false},"excerpt":{"rendered":"

Light-sheet microscopy,a brief overviewaberrationsresolutiondeeperVarious light-sheet imaging techniques differ in the way the light-sheet is created,however, they all face the same problematics regarding aberrations. These techniques are typically used to do live imaging of optically inhomogeniousbiological samples. A number of refractive index changes are then causing aberrations. This impacts PSF quality, which ultimatly will decrease image […]<\/p>\n","protected":false},"author":1,"featured_media":0,"parent":130,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_et_pb_use_builder":"on","_et_pb_old_content":"","_et_gb_content_width":"","footnotes":""},"dipi_cpt_category":[],"class_list":["post-342","page","type-page","status-publish","hentry"],"yoast_head":"\nLight-sheet microscopy, a brief overview<\/title>\n<meta name=\"description\" content=\"Few things about ligh-sheet and lattice light-sheet microscopy, plus some details on how adaptive optics could benefit these techniques.\" \/>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/mu-imagine.com\/techniques\/light-sheet-microscopy-overview\/\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta 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