{"id":352,"date":"2023-03-30T12:51:38","date_gmt":"2023-03-30T10:51:38","guid":{"rendered":"https:\/\/mu-imagine.com\/?page_id=352"},"modified":"2024-06-21T16:52:28","modified_gmt":"2024-06-21T14:52:28","slug":"multiphoton-microscopy-overview","status":"publish","type":"page","link":"https:\/\/mu-imagine.com\/techniques\/multiphoton-microscopy-overview\/","title":{"rendered":"Multiphoton"},"content":{"rendered":"

[et_pb_section fb_built=”1″ disabled_on=”off|off|off” _builder_version=”4.23.1″ _module_preset=”default” background_image=”https:\/\/mu-imagine.com\/wp-content\/uploads\/2024\/04\/Multiphoton-background.png” bottom_divider_style=”wave” bottom_divider_color=”#cbe9fb” bottom_divider_height=”70px” bottom_divider_flip=”horizontal” hover_enabled=”0″ locked=”off” global_colors_info=”{}” sticky_enabled=”0″][et_pb_row column_structure=”1_2,1_2″ _builder_version=”4.23.1″ _module_preset=”default” max_width=”1400px” custom_margin=”|auto|33px|auto||” custom_padding=”||0px|||” locked=”off” global_colors_info=”{}”][et_pb_column type=”1_2″ _builder_version=”4.23.1″ _module_preset=”default” global_colors_info=”{}”][\/et_pb_column][et_pb_column type=”1_2″ _builder_version=”4.23.1″ _module_preset=”default” global_colors_info=”{}”][et_pb_text _builder_version=”4.23.1″ _module_preset=”default” text_font=”Montserrat||||||||” text_text_color=”#FFFFFF” header_font=”Montserrat|700|||||||” header_text_color=”#cbe9fb” header_font_size=”33px” global_colors_info=”{}”]<\/p>\n

Multiphoton microscopy,
<\/span>a brief overview<\/h1>\n

[\/et_pb_text][\/et_pb_column][\/et_pb_row][et_pb_row column_structure=”1_6,1_6,1_6,1_2″ _builder_version=”4.23.1″ _module_preset=”default” max_width=”1400px” custom_padding=”0px||0px|||” locked=”off” global_colors_info=”{}”][et_pb_column type=”1_6″ _builder_version=”4.23.1″ _module_preset=”default” global_colors_info=”{}”][et_pb_blurb title=”Correct” image=”https:\/\/mu-imagine.com\/wp-content\/uploads\/2024\/04\/Correct_multiphoton.png” alt=”PSF correction icon” image_icon_width=”115px” content_max_width=”100%” module_class=”header-icons” _builder_version=”4.23.1″ _module_preset=”default” header_level=”h3″ header_font=”Montserrat|600|||||||” header_text_align=”center” header_text_color=”#FFFFFF” header_font_size=”16px” body_font=”Montserrat||||||||” body_text_align=”center” body_text_color=”#FFFFFF” body_font_size=”13px” body_line_height=”1em” image_icon_custom_margin=”0px||0px||false|false” image_icon_custom_margin_tablet=”0px||0px||false|false” image_icon_custom_margin_phone=”5px||||false|false” image_icon_custom_margin_last_edited=”on|phone” animation=”off” locked=”off” global_colors_info=”{}”]<\/p>\n

aberrations<\/p>\n

[\/et_pb_blurb][\/et_pb_column][et_pb_column type=”1_6″ _builder_version=”4.23.1″ _module_preset=”default” global_colors_info=”{}”][et_pb_blurb title=”Boost” image=”https:\/\/mu-imagine.com\/wp-content\/uploads\/2024\/04\/Boost_multiphoton.png” alt=”Fluorescence boost icon” image_icon_width=”115px” content_max_width=”100%” module_class=”header-icons” _builder_version=”4.23.1″ _module_preset=”default” header_level=”h3″ header_font=”Montserrat|600|||||||” header_text_align=”center” header_text_color=”#FFFFFF” header_font_size=”16px” body_font=”Montserrat||||||||” body_text_align=”center” body_text_color=”#FFFFFF” body_font_size=”13px” body_line_height=”1em” image_icon_custom_margin=”||0px||false|false” animation=”off” locked=”off” global_colors_info=”{}”]<\/p>\n

fluorescence<\/p>\n

[\/et_pb_blurb][\/et_pb_column][et_pb_column type=”1_6″ _builder_version=”4.23.1″ _module_preset=”default” global_colors_info=”{}”][et_pb_blurb title=”Image” image=”https:\/\/mu-imagine.com\/wp-content\/uploads\/2024\/04\/Deep_multiphoton.png” alt=”In depth imaging icon” image_icon_width=”115px” content_max_width=”100%” module_class=”header-icons” _builder_version=”4.23.1″ _module_preset=”default” header_level=”h3″ header_font=”Montserrat|600|||||||” header_text_align=”center” header_text_color=”#FFFFFF” header_font_size=”16px” body_font=”Montserrat||||||||” body_text_align=”center” body_text_color=”#FFFFFF” body_font_size=”13px” body_line_height=”1em” image_icon_custom_margin=”0px||0px||false|false” image_icon_custom_margin_tablet=”0px||0px||false|false” image_icon_custom_margin_phone=”-12px||||false|false” image_icon_custom_margin_last_edited=”on|phone” width=”100%” custom_margin_tablet=”” custom_margin_phone=”-6px||0px||false|false” custom_margin_last_edited=”on|tablet” animation=”off” locked=”off” global_colors_info=”{}”]<\/p>\n

deeper<\/p>\n

[\/et_pb_blurb][\/et_pb_column][et_pb_column type=”1_2″ _builder_version=”4.23.1″ _module_preset=”default” global_colors_info=”{}”][\/et_pb_column][\/et_pb_row][\/et_pb_section][et_pb_section fb_built=”1″ disabled_on=”off|off|off” _builder_version=”4.23.1″ _module_preset=”default” background_color=”#cbe9fb” background_enable_image=”off” background_pattern_repeat_origin=”center” hover_enabled=”0″ locked=”off” global_colors_info=”{}” sticky_enabled=”0″][et_pb_row _builder_version=”4.23.1″ _module_preset=”default” max_width=”1400px” custom_padding=”0px|||||” locked=”off” global_colors_info=”{}”][et_pb_column type=”4_4″ _builder_version=”4.23.1″ _module_preset=”default” global_colors_info=”{}”][et_pb_heading title=”About the technique” _builder_version=”4.23.1″ _module_preset=”default” title_level=”h2″ title_font=”Montserrat|700|||||||” title_text_color=”#000000″ title_font_size=”25px” custom_margin=”||-20px||false|false” custom_margin_tablet=”||-30px||false|false” custom_margin_phone=”||-30px||false|false” custom_margin_last_edited=”on|tablet” global_colors_info=”{}”][\/et_pb_heading][\/et_pb_column][\/et_pb_row][et_pb_row column_structure=”1_2,1_2″ use_custom_gutter=”on” gutter_width=”2″ make_equal=”on” _builder_version=”4.23.1″ _module_preset=”default” max_width=”1400px” custom_css_main_element=”display: flex;||align-items: center;” global_colors_info=”{}” custom_css_main_element_last_edited=”on|phone” custom_css_main_element_phone=”display: block;” custom_css_main_element_tablet=”display: block;”][et_pb_column type=”1_2″ _builder_version=”4.20.1″ _module_preset=”default” custom_css_main_element=”margin: auto;” global_colors_info=”{}”][et_pb_text _builder_version=”4.23.1″ _module_preset=”default” text_font=”Montserrat||||||||” text_text_color=”#000000″ text_font_size=”13px” header_3_font=”Montserrat|700|||||||” header_3_text_color=”#FFFFFF” header_3_font_size=”25px” custom_margin=”||35px||false|false” header_3_font_size_tablet=”25px” header_3_font_size_phone=”22px” header_3_font_size_last_edited=”on|phone” global_colors_info=”{}”]<\/p>\n

Multiphoton microscopy is usually used to image very deep inside the biological sample.<\/p>\n

Typically these are optically inhomogenious because of a number of refractive index variations which are causing aberrations.<\/p>\n

This affects the quality of the PSF, which eventually degrades image quality.<\/p>\n

[\/et_pb_text][\/et_pb_column][et_pb_column type=”1_2″ _builder_version=”4.20.1″ _module_preset=”default” global_colors_info=”{}”][et_pb_image src=”https:\/\/mu-imagine.com\/wp-content\/uploads\/2024\/04\/no-AO_Multiphoton.png” alt=”Example of multiphoton imaging without adaptive optics” title_text=”no-ao_multiphoton” align=”center” _builder_version=”4.23.1″ _module_preset=”default” max_width=”75%” max_width_phone=”75%” max_width_last_edited=”off|phone” box_shadow_style=”preset1″ global_colors_info=”{}”][\/et_pb_image][\/et_pb_column][\/et_pb_row][\/et_pb_section][et_pb_section fb_built=”1″ disabled_on=”off|off|off” _builder_version=”4.23.1″ _module_preset=”default” background_color=”#cbe9fb” hover_enabled=”0″ locked=”off” global_colors_info=”{}” sticky_enabled=”0″][et_pb_row _builder_version=”4.23.1″ _module_preset=”default” max_width=”1400px” custom_margin=”|auto|-32px|auto||” custom_padding=”0px|||||” locked=”off” global_colors_info=”{}”][et_pb_column type=”4_4″ _builder_version=”4.23.1″ _module_preset=”default” global_colors_info=”{}”][et_pb_heading title=”What can AO bring ?” _builder_version=”4.23.1″ _module_preset=”default” title_level=”h2″ title_font=”Montserrat|700|||||||” title_text_color=”#000000″ title_font_size=”25px” custom_margin=”||-25px||false|false” custom_margin_tablet=”||35px||false|false” custom_margin_phone=”||35px||false|false” custom_margin_last_edited=”on|desktop” locked=”off” global_colors_info=”{}”][\/et_pb_heading][\/et_pb_column][\/et_pb_row][et_pb_row column_structure=”1_2,1_2″ make_equal=”on” _builder_version=”4.23.1″ _module_preset=”default” max_width=”1400px” custom_margin=”|auto|-65px|auto|false|false” custom_padding=”0px||0px||false|false” custom_css_main_element=”display: flex;||align-items: center;” global_colors_info=”{}” custom_css_main_element_last_edited=”on|phone” custom_css_main_element_tablet=”display: block;” custom_css_main_element_phone=”display: block;”][et_pb_column type=”1_2″ _builder_version=”4.23.1″ _module_preset=”default” global_colors_info=”{}”][et_pb_blurb use_icon=”on” font_icon=”R||divi||400″ icon_color=”#02486A” icon_placement=”left” image_icon_width=”22px” content_max_width=”100%” _builder_version=”4.23.1″ _module_preset=”default” body_font=”Montserrat||||||||” body_text_color=”#000000″ body_font_size=”13px” body_line_height=”1.3em” custom_margin=”||10px||false|false” animation=”off” locked=”off” global_colors_info=”{}”]<\/p>\n

Implement on excitation pathway <\/b>and boost significantly the fluorescence signal from your biological sample<\/p>\n

[\/et_pb_blurb][et_pb_blurb use_icon=”on” font_icon=”R||divi||400″ icon_color=”#02486A” icon_placement=”left” image_icon_width=”22px” content_max_width=”100%” _builder_version=”4.23.1″ _module_preset=”default” body_font=”Montserrat||||||||” body_text_color=”#000000″ body_font_size=”13px” body_line_height=”1.3em” custom_margin=”||10px||false|false” animation=”off” locked=”off” global_colors_info=”{}”]<\/p>\n

Correct the system and sample-induced aberrations <\/b>with a typical gain of 3 to 5 times the two-photon effect<\/p>\n

[\/et_pb_blurb][et_pb_blurb use_icon=”on” font_icon=”R||divi||400″ icon_color=”#02486A” icon_placement=”left” image_icon_width=”22px” content_max_width=”100%” _builder_version=”4.23.1″ _module_preset=”default” body_font=”Montserrat||||||||” body_text_color=”#000000″ body_font_size=”13px” body_line_height=”1.3em” custom_margin=”||10px||false|false” animation=”off” locked=”off” global_colors_info=”{}”]<\/p>\n

Increase fluorescence signal <\/b>and either reach deeper layers of your sample or decrease excitation laser intensity to minimize photo-toxicity<\/p>\n

[\/et_pb_blurb][\/et_pb_column][et_pb_column type=”1_2″ _builder_version=”4.23.1″ _module_preset=”default” global_colors_info=”{}”][et_pb_image src=”https:\/\/mu-imagine.com\/wp-content\/uploads\/2024\/04\/AO_Multiphoton.png” alt=”Example of aberration correction with adaptive optics on multiphoton imaging” title_text=”ao-multiphoton” align=”center” _builder_version=”4.23.1″ _module_preset=”default” width_tablet=”75%” width_last_edited=”off|desktop” max_width=”75%” box_shadow_style=”preset1″ global_colors_info=”{}”][\/et_pb_image][\/et_pb_column][\/et_pb_row][et_pb_row column_structure=”1_2,1_2″ use_custom_gutter=”on” gutter_width=”2″ _builder_version=”4.23.1″ _module_preset=”default” max_width=”1400px” custom_padding=”0px||0px||true|false” locked=”off” global_colors_info=”{}” custom_css_main_element_last_edited=”off|desktop” custom_css_main_element_phone=”display: block;” custom_css_main_element_tablet=”display: flex;”][et_pb_column type=”1_2″ _builder_version=”4.20.1″ _module_preset=”default” custom_css_main_element=”margin: auto;” global_colors_info=”{}”][et_pb_text _builder_version=”4.23.1″ _module_preset=”default” text_font=”Montserrat|700|||||||” text_text_color=”#02486A” text_font_size=”16px” header_3_font=”Montserrat|700|||||||” header_3_text_color=”#000000″ header_3_font_size=”25px” header_4_font=”Montserrat|700|||||||” header_4_text_color=”#1b375c” header_4_font_size=”20px” custom_margin=”35px||-11px||false|false” header_3_font_size_tablet=”25px” header_3_font_size_phone=”22px” header_3_font_size_last_edited=”on|phone” global_colors_info=”{}”]<\/p>\n

Related products<\/h4>\n

[\/et_pb_text][dipi_button_grid flex_direction=”row” justify_content=”flex-start” _builder_version=”4.23.1″ _module_preset=”default” text_style_text_color=”#000000″ custom_button_grid=”on” button_grid_text_size=”16px” button_grid_text_color=”#000000″ button_grid_bg_color=”#0cb8dc” button_grid_border_width=”0px” button_grid_font=”Montserrat|600|||||||” hover_enabled=”0″ global_colors_info=”{}” sticky_enabled=”0″][dipi_button_grid_child button_type=”button” button_text=”AOKit Bio” button_link=”@ET-DC@eyJkeW5hbWljIjp0cnVlLCJjb250ZW50IjoicG9zdF9saW5rX3VybF9wYWdlIiwic2V0dGluZ3MiOnsicG9zdF9pZCI6IjIwMiJ9fQ==@” _builder_version=”4.23.1″ _dynamic_attributes=”button_link” _module_preset=”default” custom_button=”on” button_text_size=”16px” button_text_color=”#000000″ button_bg_color=”#0cb8dc” button_border_width=”0px” button_border_color=”#50D3E1″ button_border_radius=”100px” button_font=”Montserrat|600|||||||” button_icon=”$||divi||400″ button_icon_color=”#000000″ button_on_hover=”on” hover_enabled=”0″ global_colors_info=”{}” button_bg_enable_color=”on” sticky_enabled=”0″][\/dipi_button_grid_child][dipi_button_grid_child button_type=”button” button_text=”mu-DM” button_link=”@ET-DC@eyJkeW5hbWljIjp0cnVlLCJjb250ZW50IjoicG9zdF9saW5rX3VybF9wYWdlIiwic2V0dGluZ3MiOnsicG9zdF9pZCI6IjE0NiJ9fQ==@” _builder_version=”4.23.1″ _dynamic_attributes=”button_link” _module_preset=”default” custom_button=”on” button_text_size=”16px” button_text_color=”#000000″ button_bg_color=”#0cb8dc” button_border_width=”0px” button_border_color=”#50D3E1″ button_border_radius=”100px” button_font=”Montserrat|600|||||||” button_icon=”$||divi||400″ button_icon_color=”#000000″ button_on_hover=”on” hover_enabled=”0″ global_colors_info=”{}” button_bg_enable_color=”on” sticky_enabled=”0″][\/dipi_button_grid_child][dipi_button_grid_child button_type=”button” button_text=”Mirao” button_link=”@ET-DC@eyJkeW5hbWljIjp0cnVlLCJjb250ZW50IjoicG9zdF9saW5rX3VybF9wYWdlIiwic2V0dGluZ3MiOnsicG9zdF9pZCI6IjM3MiJ9fQ==@” _builder_version=”4.23.1″ _dynamic_attributes=”button_link” _module_preset=”default” custom_button=”on” button_text_size=”16px” button_text_color=”#000000″ button_bg_color=”#0cb8dc” button_border_radius=”100px” button_font=”Montserrat|600|||||||” button_icon=”$||divi||400″ button_icon_color=”#000000″ button_on_hover=”on” hover_enabled=”0″ global_colors_info=”{}” sticky_enabled=”0″][\/dipi_button_grid_child][\/dipi_button_grid][\/et_pb_column][et_pb_column type=”1_2″ _builder_version=”4.20.1″ _module_preset=”default” global_colors_info=”{}”][\/et_pb_column][\/et_pb_row][\/et_pb_section][et_pb_section fb_built=”1″ disabled_on=”off|off|off” _builder_version=”4.23.1″ _module_preset=”default” background_color=”#cbe9fb” bottom_divider_style=”wave” bottom_divider_color=”#0e0a0f” bottom_divider_height=”70px” hover_enabled=”0″ locked=”off” global_colors_info=”{}” sticky_enabled=”0″][et_pb_row use_custom_gutter=”on” gutter_width=”2″ _builder_version=”4.20.1″ _module_preset=”default” max_width=”1400px” custom_padding=”0px||0px||true|false” custom_css_main_element=”display: flex;” global_colors_info=”{}” custom_css_main_element_tablet=”display: flex;” custom_css_main_element_phone=”display: block;” custom_css_main_element_last_edited=”on|phone”][et_pb_column type=”4_4″ _builder_version=”4.20.1″ _module_preset=”default” global_colors_info=”{}”][et_pb_heading title=”Applications examples” _builder_version=”4.23.1″ _module_preset=”default” title_level=”h2″ title_font=”Montserrat|700|||||||” title_text_color=”#000000″ title_font_size=”25px” locked=”off” global_colors_info=”{}”][\/et_pb_heading][et_pb_text _builder_version=”4.23.1″ _module_preset=”default” text_font=”Montserrat||||||||” text_text_color=”#000000″ text_font_size=”13px” global_colors_info=”{}”]<\/p>\n

Application notes & white papers coming soon<\/p>\n

[\/et_pb_text][et_pb_text _builder_version=”4.23.1″ _module_preset=”default” text_font=”Montserrat||||||||” text_text_color=”#DDE4E6″ text_font_size=”16px” header_3_font=”Montserrat|700|||||||” header_3_text_color=”#50D3E1″ header_3_font_size=”25px” header_4_font=”Montserrat|700|||||||” header_4_text_color=”#1b375c” header_4_font_size=”20px” custom_margin=”35px||35px||false|false” header_3_font_size_tablet=”25px” header_3_font_size_phone=”22px” header_3_font_size_last_edited=”on|phone” header_4_font_size_tablet=”18px” header_4_font_size_phone=”18px” header_4_font_size_last_edited=”off|desktop” locked=”off” global_colors_info=”{}”]<\/p>\n

Selected publications<\/h4>\n

N. Olivier, D. D\u00e9barre & E. Beaurepaire; “Dynamic aberration correction for multiharmonic microscopy”; Opt. Lett. 34, 3145-3147 (2009).<\/a><\/p>\n

B. Sun, P. S. Salter & M. J. Booth; “Pulse front adaptive optics in twophoton microscopy”; Opt. Lett. 40, 4999-5002 (2015).<\/a><\/p>\n

W. Zheng, Y. Wu, P. Winter & al.; “Adaptive optics improves multiphoton super-resolution imaging”; Nat Methods 14, 869\u2013872 (2017).<\/a>[\/et_pb_text][\/et_pb_column][\/et_pb_row][\/et_pb_section]<\/p>\n","protected":false},"excerpt":{"rendered":"

Multiphoton microscopy,a brief overviewaberrationsfluorescencedeeperMultiphoton microscopy is usually used to image very deep inside the biological sample. Typically these are optically inhomogenious because of a number of refractive index variations which are causing aberrations. This affects the quality of the PSF, which eventually degrades image quality.Implement on excitation pathway and boost significantly the fluorescence signal from […]<\/p>\n","protected":false},"author":1,"featured_media":0,"parent":130,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_et_pb_use_builder":"on","_et_pb_old_content":"","_et_gb_content_width":"","footnotes":""},"dipi_cpt_category":[],"class_list":["post-352","page","type-page","status-publish","hentry"],"yoast_head":"\nMultiphoton microscopy, a brief overview<\/title>\n<meta name=\"description\" content=\"A quick overview of multiphoton microscopy, plus some details on how adaptive optics (AO) could benefit it.\" \/>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/mu-imagine.com\/techniques\/multiphoton-microscopy-overview\/\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Multiphoton microscopy, a brief overview\" \/>\n<meta property=\"og:description\" content=\"A quick overview of multiphoton microscopy, plus some details on how adaptive optics (AO) could benefit it.\" \/>\n<meta property=\"og:url\" content=\"https:\/\/mu-imagine.com\/techniques\/multiphoton-microscopy-overview\/\" \/>\n<meta property=\"og:site_name\" content=\"mu-Imagine\" \/>\n<meta property=\"article:modified_time\" content=\"2024-06-21T14:52:28+00:00\" \/>\n<meta name=\"twitter:card\" content=\"summary_large_image\" \/>\n<meta name=\"twitter:site\" content=\"@mu_imagine\" \/>\n<meta name=\"twitter:label1\" content=\"Est. reading time\" \/>\n\t<meta name=\"twitter:data1\" content=\"3 minutes\" \/>\n<script type=\"application\/ld+json\" class=\"yoast-schema-graph\">{\"@context\":\"https:\/\/schema.org\",\"@graph\":[{\"@type\":\"WebPage\",\"@id\":\"https:\/\/mu-imagine.com\/techniques\/multiphoton-microscopy-overview\/\",\"url\":\"https:\/\/mu-imagine.com\/techniques\/multiphoton-microscopy-overview\/\",\"name\":\"Multiphoton microscopy, a brief overview\",\"isPartOf\":{\"@id\":\"https:\/\/mu-imagine.com\/#website\"},\"datePublished\":\"2023-03-30T10:51:38+00:00\",\"dateModified\":\"2024-06-21T14:52:28+00:00\",\"description\":\"A quick overview of multiphoton microscopy, plus some details on how adaptive optics (AO) could benefit it.\",\"breadcrumb\":{\"@id\":\"https:\/\/mu-imagine.com\/techniques\/multiphoton-microscopy-overview\/#breadcrumb\"},\"inLanguage\":\"en-US\",\"potentialAction\":[{\"@type\":\"ReadAction\",\"target\":[\"https:\/\/mu-imagine.com\/techniques\/multiphoton-microscopy-overview\/\"]}]},{\"@type\":\"BreadcrumbList\",\"@id\":\"https:\/\/mu-imagine.com\/techniques\/multiphoton-microscopy-overview\/#breadcrumb\",\"itemListElement\":[{\"@type\":\"ListItem\",\"position\":1,\"name\":\"Home\",\"item\":\"https:\/\/mu-imagine.com\/\"},{\"@type\":\"ListItem\",\"position\":2,\"name\":\"Techniques\",\"item\":\"https:\/\/mu-imagine.com\/techniques\/\"},{\"@type\":\"ListItem\",\"position\":3,\"name\":\"Multiphoton\"}]},{\"@type\":\"WebSite\",\"@id\":\"https:\/\/mu-imagine.com\/#website\",\"url\":\"https:\/\/mu-imagine.com\/\",\"name\":\"mu-Imagine\",\"description\":\"Adaptive optics, adapted to microscopy\",\"publisher\":{\"@id\":\"https:\/\/mu-imagine.com\/#organization\"},\"potentialAction\":[{\"@type\":\"SearchAction\",\"target\":{\"@type\":\"EntryPoint\",\"urlTemplate\":\"https:\/\/mu-imagine.com\/?s={search_term_string}\"},\"query-input\":\"required name=search_term_string\"}],\"inLanguage\":\"en-US\"},{\"@type\":\"Organization\",\"@id\":\"https:\/\/mu-imagine.com\/#organization\",\"name\":\"mu-Imagine\",\"url\":\"https:\/\/mu-imagine.com\/\",\"logo\":{\"@type\":\"ImageObject\",\"inLanguage\":\"en-US\",\"@id\":\"https:\/\/mu-imagine.com\/#\/schema\/logo\/image\/\",\"url\":\"https:\/\/mu-imagine.com\/wp-content\/uploads\/2023\/03\/cropped-Logo-muImagine.png\",\"contentUrl\":\"https:\/\/mu-imagine.com\/wp-content\/uploads\/2023\/03\/cropped-Logo-muImagine.png\",\"width\":512,\"height\":512,\"caption\":\"mu-Imagine\"},\"image\":{\"@id\":\"https:\/\/mu-imagine.com\/#\/schema\/logo\/image\/\"},\"sameAs\":[\"https:\/\/twitter.com\/mu_imagine\",\"https:\/\/www.linkedin.com\/company\/mu-imagine\/\"]}]}<\/script>\n<!-- \/ Yoast SEO plugin. -->","yoast_head_json":{"title":"Multiphoton microscopy, a brief overview","description":"A quick overview of multiphoton microscopy, plus some details on how adaptive optics (AO) could benefit it.","robots":{"index":"index","follow":"follow","max-snippet":"max-snippet:-1","max-image-preview":"max-image-preview:large","max-video-preview":"max-video-preview:-1"},"canonical":"https:\/\/mu-imagine.com\/techniques\/multiphoton-microscopy-overview\/","og_locale":"en_US","og_type":"article","og_title":"Multiphoton microscopy, a brief overview","og_description":"A quick overview of multiphoton microscopy, plus some details on how adaptive optics (AO) could benefit it.","og_url":"https:\/\/mu-imagine.com\/techniques\/multiphoton-microscopy-overview\/","og_site_name":"mu-Imagine","article_modified_time":"2024-06-21T14:52:28+00:00","twitter_card":"summary_large_image","twitter_site":"@mu_imagine","twitter_misc":{"Est. reading time":"3 minutes"},"schema":{"@context":"https:\/\/schema.org","@graph":[{"@type":"WebPage","@id":"https:\/\/mu-imagine.com\/techniques\/multiphoton-microscopy-overview\/","url":"https:\/\/mu-imagine.com\/techniques\/multiphoton-microscopy-overview\/","name":"Multiphoton microscopy, a brief overview","isPartOf":{"@id":"https:\/\/mu-imagine.com\/#website"},"datePublished":"2023-03-30T10:51:38+00:00","dateModified":"2024-06-21T14:52:28+00:00","description":"A quick overview of multiphoton microscopy, plus some details on how adaptive optics (AO) could benefit it.","breadcrumb":{"@id":"https:\/\/mu-imagine.com\/techniques\/multiphoton-microscopy-overview\/#breadcrumb"},"inLanguage":"en-US","potentialAction":[{"@type":"ReadAction","target":["https:\/\/mu-imagine.com\/techniques\/multiphoton-microscopy-overview\/"]}]},{"@type":"BreadcrumbList","@id":"https:\/\/mu-imagine.com\/techniques\/multiphoton-microscopy-overview\/#breadcrumb","itemListElement":[{"@type":"ListItem","position":1,"name":"Home","item":"https:\/\/mu-imagine.com\/"},{"@type":"ListItem","position":2,"name":"Techniques","item":"https:\/\/mu-imagine.com\/techniques\/"},{"@type":"ListItem","position":3,"name":"Multiphoton"}]},{"@type":"WebSite","@id":"https:\/\/mu-imagine.com\/#website","url":"https:\/\/mu-imagine.com\/","name":"mu-Imagine","description":"Adaptive optics, adapted to microscopy","publisher":{"@id":"https:\/\/mu-imagine.com\/#organization"},"potentialAction":[{"@type":"SearchAction","target":{"@type":"EntryPoint","urlTemplate":"https:\/\/mu-imagine.com\/?s={search_term_string}"},"query-input":"required name=search_term_string"}],"inLanguage":"en-US"},{"@type":"Organization","@id":"https:\/\/mu-imagine.com\/#organization","name":"mu-Imagine","url":"https:\/\/mu-imagine.com\/","logo":{"@type":"ImageObject","inLanguage":"en-US","@id":"https:\/\/mu-imagine.com\/#\/schema\/logo\/image\/","url":"https:\/\/mu-imagine.com\/wp-content\/uploads\/2023\/03\/cropped-Logo-muImagine.png","contentUrl":"https:\/\/mu-imagine.com\/wp-content\/uploads\/2023\/03\/cropped-Logo-muImagine.png","width":512,"height":512,"caption":"mu-Imagine"},"image":{"@id":"https:\/\/mu-imagine.com\/#\/schema\/logo\/image\/"},"sameAs":["https:\/\/twitter.com\/mu_imagine","https:\/\/www.linkedin.com\/company\/mu-imagine\/"]}]}},"_links":{"self":[{"href":"https:\/\/mu-imagine.com\/wp-json\/wp\/v2\/pages\/352","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/mu-imagine.com\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/mu-imagine.com\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/mu-imagine.com\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/mu-imagine.com\/wp-json\/wp\/v2\/comments?post=352"}],"version-history":[{"count":13,"href":"https:\/\/mu-imagine.com\/wp-json\/wp\/v2\/pages\/352\/revisions"}],"predecessor-version":[{"id":1051,"href":"https:\/\/mu-imagine.com\/wp-json\/wp\/v2\/pages\/352\/revisions\/1051"}],"up":[{"embeddable":true,"href":"https:\/\/mu-imagine.com\/wp-json\/wp\/v2\/pages\/130"}],"wp:attachment":[{"href":"https:\/\/mu-imagine.com\/wp-json\/wp\/v2\/media?parent=352"}],"wp:term":[{"taxonomy":"dipi_cpt_category","embeddable":true,"href":"https:\/\/mu-imagine.com\/wp-json\/wp\/v2\/dipi_cpt_category?post=352"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}