AOkit BIO, an adaptive optics kit
tailored to bioimaging

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Image of AOkit BIO, the adaptive optics kit
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Add AO

to your microscope

Controllers icon

Control the PSF

of your optical setup

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Use a software

adapted to your needs

Description

Construction of adaptive optics kit
AOKit Bio is a set of Adaptive Optics components, including a Shack-Hartmann wavefront sensor (among HASO family), a deformable mirror or phase modulator, as well as an adaptive optics software (SDK or software with user interface).

AOKit Bio allows you to build the adaptive optics system that best suits your individual needs.

Technology & features

With which techniques can you use it ?

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Multiphoton microscopy

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Light-sheet microscopy

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Lattice light-sheet microscopy

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PALM / STORM

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STED microscopy

Various microscopy set-ups on which adaptive optics kit was implemented

Advantages of AOkit BIO

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Choose your elements depending on your needs : AO loop can be integrated with different phase modulators, such as deformable mirrors or spatial light modulators (SLM)

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Double the number of detected photons

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Operate in both closed and open loop mode

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Save time implementing your AO set-up

AOkit BIO possible components

About the software

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For easy and fast implementation, we recommend using WaveTune, program controls all the elements with a simple user interface

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For implementation of aberration detection methods into home-built software, we also provide WaveKit Bio, the Software Development Kit (SDK)

Laptop with software screenshot

Gallery

Z stack AO-2 Photon imaging of GADGFP mouse brain slices, inhibitory neurons labelled; Courtesy of S. Imperato, L. Bourdieu (LPEM, ENS-IBENS, Imagine Optic)
Max. intensity projections of a small stack of neuronal projections at 32 µm depth imaged using image-based (3N) AOLattice Light-Sheet; Courtesy of M. Malivert (BIC)
Neuronal cells in the zebrafish at 180-300 µm depth using AO-ASLM; Courtesy of M. Mercier, W. Supatto (ISMO, LOB)

Selected publications

K.M. Dean, P. Roudot, E.S. Welf, T. Pohlkamp, G. Garrelts, J. Herz, & R. Fiolka; “Imaging subcellular dynamics with fast and light-efficient volumetrically parallelized microscopy”; Optica 4, 263-271 (2017)

W. Zheng, Y. Wu, P. Winter & al.; “Adaptive optics improves multiphoton super-resolution imaging”; Nat Methods 14, 869–872 (2017)

R. Chowdhury, A. Sau & S.M. Musser; “Super-resolved 3D tracking of cargo transport through nuclear pore complexes”; Nat Cell Biol 24, 112–122 (2022)

Discover techniques for which AO applies

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