Single Molecule Localization Microscopy







About the technique

In order to fully benefit from the resolving power of single molecule localization techniques, it is very important to minimize aberrations that arise in real life situations. The microscope’s components, the refractive index mismatch between the objective and the immersion medium, and the biological sample itself can all introduce various types of aberrations. This results in a degradation of the PSF’s quality which leads to a reduction in the number of detected photons and, ultimately, a reduction in resolution.

What can AO bring ?

Perfect the PSF Increase number of photons & localization precision in 2D Restore PSF axial symmetry Increase 3D localization precision by restoring symmetry of the calibration curve Benefit from various PSF shaping capabilities Astigmatism, Tetrapod

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