Multiphoton microscopy,
a brief overview

PSF correction icon

Correct

aberrations

Fluorescence boost icon

Boost

fluorescence

In depth imaging icon

Image

deeper

About the technique

Multiphoton microscopy is usually used to image very deep inside the biological sample.

Typically these are optically inhomogenious because of a number of refractive index variations which are causing aberrations.

This affects the quality of the PSF, which eventually degrades image quality.

Example of multiphoton imaging without adaptive optics

What can AO bring ?

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Implement on excitation pathway and boost significantly the fluorescence signal from your biological sample

R

Correct the system and sample-induced aberrations with a typical gain of 3 to 5 times the two-photon effect

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Increase fluorescence signal and either reach deeper layers of your sample or decrease excitation laser intensity to minimize photo-toxicity

Example of aberration correction with adaptive optics on multiphoton imaging

Related products

AOKit Bio
mu-DM
Mirao

Applications examples

Application notes & white papers coming soon

Selected publications

N. Olivier, D. Débarre & E. Beaurepaire; “Dynamic aberration correction for multiharmonic microscopy”; Opt. Lett. 34, 3145-3147 (2009).

B. Sun, P. S. Salter & M. J. Booth; “Pulse front adaptive optics in twophoton microscopy”; Opt. Lett. 40, 4999-5002 (2015).

W. Zheng, Y. Wu, P. Winter & al.; “Adaptive optics improves multiphoton super-resolution imaging”; Nat Methods 14, 869–872 (2017).