MicAO, an adaptive optics module
for super resolution microscopy

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Increase resolution

by correcting aberrations

Shape your PSF

and go 3D

Image deeper

up to 50µm depth

Description

MicAO Adaptive Optics (AO) device is the first AO add-on module dedicated to techniques such as PhotoActivation Localization Microscopy (PALM), Stochastic Optical Reconstruction Microscopy (STORM) and Single Particle Tracking (SPT).

This module will enable you to get a better resolution in both 2D&3D for Single Molecule Localization Microscopy (SMLM).

Technology & features

How is it implemented ?

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Added between the side port of your inverted frame microscope and the imaging camera

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Functions as an image relay : allows you to add other modules (dual color view device, microscope port splitters…)

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Compatible with 60x and 100x objective lenses

MicAO adaptive optics module inserted in inverted microscope side port

Advantages of MicAO

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Restores Point Spread Function (PSF) symmetry

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Doubles the number of detected photons

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Improves near diffraction-limited resolution

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Allows you to create a perfect PSF ecoding astigmatism or tetrapod to go 3D

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Gives you a better 2D and 3D localization precision

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Is compatible with long-term imaging

PSF before and after correction with MicAO

How does it work ?

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Enables you to function in open-loop configuration

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Helps you to apply 3N alogorithm to detect and correct aberrations, thanks to MicAO software

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Allows you to control your mirror and shape your PSF

Gallery

Example images of the diffraction-limited fluorescent bead at different ambient temperatures without stabilization module inside MicAO.

Combined two-color 3 dimensional PALM and dSTORM imaging of mEos2-Centrin1 (orange) and Alexa 647 labeled Cep164 (light blue). Vizualisation using VISP sofware (El Beheiry and Dahan, 2013). Courtesy of J. Sillibourne & I. Izeddin

Comparison of astigmatism induced by cylindrical lens (left) and deformable mirror (right). We can see increased localization number with the DM, a component of MicAO.