Light-sheet microscopy,
a brief overview

PSF correction icon

Correct

aberrations

Resolution improvement icon

Improve

resolution

In depth imaging icon

Image

deeper

About the technique

Various light-sheet imaging techniques differ in the way the light-sheet is created,
however, they all face the same problematics regarding aberrations.

These techniques are typically used to do live imaging of optically inhomogenious
biological samples. A number of refractive index changes are then causing aberrations.

This impacts PSF quality, which ultimatly will decrease image contrast.

Examples of light-sheet and lattice light-sheet imaging without adaptive optics

What can AO bring ?

R

Implement on emission pathway, increase fluorescence signal and contrast of the image

R

Correct the system aberrations induced by optical elements and sample mounting

R

Correct sample-induced aberrations and see biological structures more clearly

Examples of light-sheet and lattice light-sheet imaging with adaptive optics

Related products

AOKit Bio
mu-DM
Mirao

Applications examples

Application notes & white papers coming soon

Selected publications

C. Bourgenot, C. D. Saunter, J. M. Taylor, J. M. Girkin & G. D. Love; “3D adaptive optics in a light sheet microscope”; Opt. Express 20, 13252-13261 (2012)

K.M. Dean, P. Roudot, E.S. Welf, T. Pohlkamp, G. Garrelts, J. Herz, & R. Fiolka; “Imaging subcellular dynamics with fast and light-efficient volumetrically parallelized microscopy”; Optica 4, 263-271 (2017).

A. Hubert, F. Harms, R. Juvénal, P. Treimany, X. Levecq, V. Loriette, G. Farkouh, F. Rouyer & A. Fragola; “Adaptive optics light-sheet microscopy based on direct wavefront sensing without any guide star”; Opt. Lett. 44, 2514-2517 (2019)