About the technique
Multiphoton microscopy is usually used to image very deep inside the biological sample. Typically these are optically inhomogenious because of a number of refractive index variations which are causing aberrations. This affects the quality of the PSF, which eventually degrades image quality.
What can AO bring ?
Implement on excitation pathway and boost significantly the fluorescence signal from your biological sample Correct the system and sample-induced aberrations with a typical gain of 3 to 5 times the two-photon effect Increase fluorescence signal and either reach deeper layers of your sample or decrease excitation laser intensity to minimize photo-toxicity
Application & Technical notes coming soon