Single Molecule Localization Microscopy

In order to fully benefit from the resolving power of single molecule localization techniques, it is very important to minimize aberrations that arise in real life situations. The microscope’s components, the refractive index mismatch between the objective and the immersion medium, and the biological sample itself can all introduce various types of aberrations. This results in a degradation of the PSF’s quality which leads to a reduction in the number of detected photons and, ultimately, a reduction in resolution.